Journal: ACS Applied Materials & Interfaces

Article Title: Long-Term Controlled Growth Factor Release Using Layer-by-Layer Assembly for the Development of In Vivo Tissue-Engineered Blood Vessels

doi: 10.1021/acsami.2c05988

Figure Lengend Snippet: General overview of the cellular composition of the tissue capsule formed around implanted rods. (A) vWF staining for vascularization, (B) Ki67 for proliferating cells, (C) α-SMA for myofibroblast, (D) desmin for contractile smooth muscle cells, and (E) vimentin for fibroblast. The tissue capsules are mainly composed of fibroblasts and myofibroblasts. There are barely contractile smooth muscle cells present. All tissue capsules are well vascularized. Scale bar represents 50 μm.

Article Snippet: The cellular composition was evaluated with antibodies against α-smooth muscle actin (α-SMA, Dako, The Netherlands, 1:1000) for myofibroblasts, vimentin (Thermo Scientific, The Netherlands, 1:20 heat-induced citrate antigen retrieval) for fibroblasts, desmin (Thermo Scientific, The Netherlands, 1:50, heat-induced 0.1% trypsin antigen retrieval) for contractile smooth muscle cells, CD45 (Immunologic, The Netherlands, 1:150, heat-induced 0.1% trypsin antigen retrieval) for leukocytes, Von Willebrand factor (Dako, The Netherlands, 1:500, heat-induced 0.1% trypsin antigen retrieval) for endothelial cells, and Ki67 (BD Pharmingen, The Netherlands, 1:20, heat-induced citrate antigen retrieval) for proliferating cells, and visualized with 3,3′-diaminobenzadine (DAB).

Techniques: Staining